PbrSLAH3 is a nitrate-selective anion channel which is modulated by calcium-dependent protein kinase 32 in pear.
Identifieur interne : 000800 ( Main/Exploration ); précédent : 000799; suivant : 000801PbrSLAH3 is a nitrate-selective anion channel which is modulated by calcium-dependent protein kinase 32 in pear.
Auteurs : Guodong Chen [République populaire de Chine] ; Li Wang [République populaire de Chine] ; Qian Chen [République populaire de Chine] ; Kaijie Qi [République populaire de Chine] ; Hao Yin [République populaire de Chine] ; Peng Cao [République populaire de Chine] ; Chao Tang [République populaire de Chine] ; Xiao Wu [République populaire de Chine] ; Shaoling Zhang [République populaire de Chine] ; Peng Wang [République populaire de Chine] ; Juyou Wu [République populaire de Chine]Source :
- BMC plant biology [ 1471-2229 ] ; 2019.
Descripteurs français
- KwdFr :
- Animaux (MeSH), Arabidopsis (effets des médicaments et des substances chimiques), Arabidopsis (génétique), Canaux ioniques (métabolisme), Composés d'ammonium (toxicité), Gènes de plante (MeSH), Mutation (génétique), Nitrates (métabolisme), Ovocytes (effets des médicaments et des substances chimiques), Ovocytes (métabolisme), Phylogenèse (MeSH), Phénomènes électrophysiologiques (MeSH), Protein kinases (métabolisme), Protéines végétales (génétique), Protéines végétales (métabolisme), Pyrus (effets des médicaments et des substances chimiques), Pyrus (enzymologie), Pyrus (génétique), Régulation de l'expression des gènes végétaux (effets des médicaments et des substances chimiques), Végétaux génétiquement modifiés (MeSH), Xenopus (MeSH).
- MESH :
- effets des médicaments et des substances chimiques : Arabidopsis, Ovocytes, Pyrus, Régulation de l'expression des gènes végétaux.
- enzymologie : Pyrus.
- génétique : Arabidopsis, Mutation, Protéines végétales, Pyrus.
- métabolisme : Canaux ioniques, Nitrates, Ovocytes, Protein kinases, Protéines végétales.
- toxicité : Composés d'ammonium.
- Animaux, Gènes de plante, Phylogenèse, Phénomènes électrophysiologiques, Végétaux génétiquement modifiés, Xenopus.
English descriptors
- KwdEn :
- Ammonium Compounds (toxicity), Animals (MeSH), Arabidopsis (drug effects), Arabidopsis (genetics), Electrophysiological Phenomena (MeSH), Gene Expression Regulation, Plant (drug effects), Genes, Plant (MeSH), Ion Channels (metabolism), Mutation (genetics), Nitrates (metabolism), Oocytes (drug effects), Oocytes (metabolism), Phylogeny (MeSH), Plant Proteins (genetics), Plant Proteins (metabolism), Plants, Genetically Modified (MeSH), Protein Kinases (metabolism), Pyrus (drug effects), Pyrus (enzymology), Pyrus (genetics), Xenopus (MeSH).
- MESH :
- chemical , genetics : Plant Proteins.
- chemical , metabolism : Ion Channels, Nitrates, Plant Proteins, Protein Kinases.
- chemical , toxicity : Ammonium Compounds.
- drug effects : Arabidopsis, Gene Expression Regulation, Plant, Oocytes, Pyrus.
- enzymology : Pyrus.
- genetics : Arabidopsis, Mutation, Pyrus.
- metabolism : Oocytes.
- Animals, Electrophysiological Phenomena, Genes, Plant, Phylogeny, Plants, Genetically Modified, Xenopus.
Abstract
BACKGROUND
The functional characteristics of SLAC/SLAH family members isolated from Arabidopsis thaliana, poplar, barley and rice have been comprehensively investigated. However, there are no reports regarding SLAC/SLAH family genes from Rosaceae plants.
RESULTS
In this study, the function of PbrSLAH3, which is predominately expressed in pear (Pyrus bretschneideri) root, was investigated. PbrSLAH3 can rescue the ammonium toxicity phenomenon of slah3 mutant plants under high-ammonium/low-nitrate conditions. In addition, yeast two-hybrid and bimolecular fluorescence complementation assays confirmed that PbrSLAH3 interacts with PbrCPK32. Moreover, when PbrSLAH3 was co-expressed with either the Arabidopsis calcium-dependent protein kinase (CPK) 21 or PbrCPK32 in Xenopus oocytes, yellow fluorescence was emitted from the oocytes and typical anion currents were recorded in the presence of extracellular NO
CONCLUSION
We suggest that PbrSLAH3 crossing-talk with PbrCPK32 probably participate in transporting of nitrate nutrition in pear root.
DOI: 10.1186/s12870-019-1813-z
PubMed: 31068146
PubMed Central: PMC6507222
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Tongwei Road, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Cao, Peng" sort="Cao, Peng" uniqKey="Cao P" first="Peng" last="Cao">Peng Cao</name><affiliation wicri:level="1"><nlm:affiliation>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Tang, Chao" sort="Tang, Chao" uniqKey="Tang C" first="Chao" last="Tang">Chao Tang</name><affiliation wicri:level="1"><nlm:affiliation>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Xiao" sort="Wu, Xiao" uniqKey="Wu X" first="Xiao" last="Wu">Xiao Wu</name><affiliation wicri:level="1"><nlm:affiliation>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Shaoling" sort="Zhang, Shaoling" uniqKey="Zhang S" first="Shaoling" last="Zhang">Shaoling Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. 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Tongwei Road, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Juyou" sort="Wu, Juyou" uniqKey="Wu J" first="Juyou" last="Wu">Juyou Wu</name><affiliation wicri:level="1"><nlm:affiliation>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing, China. juyouwu@njau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. 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Tongwei Road, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Li" sort="Wang, Li" uniqKey="Wang L" first="Li" last="Wang">Li Wang</name><affiliation wicri:level="1"><nlm:affiliation>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Qian" sort="Chen, Qian" uniqKey="Chen Q" first="Qian" last="Chen">Qian Chen</name><affiliation wicri:level="1"><nlm:affiliation>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Qi, Kaijie" sort="Qi, Kaijie" uniqKey="Qi K" first="Kaijie" last="Qi">Kaijie Qi</name><affiliation wicri:level="1"><nlm:affiliation>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Yin, Hao" sort="Yin, Hao" uniqKey="Yin H" first="Hao" last="Yin">Hao Yin</name><affiliation wicri:level="1"><nlm:affiliation>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Cao, Peng" sort="Cao, Peng" uniqKey="Cao P" first="Peng" last="Cao">Peng Cao</name><affiliation wicri:level="1"><nlm:affiliation>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Tang, Chao" sort="Tang, Chao" uniqKey="Tang C" first="Chao" last="Tang">Chao Tang</name><affiliation wicri:level="1"><nlm:affiliation>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Xiao" sort="Wu, Xiao" uniqKey="Wu X" first="Xiao" last="Wu">Xiao Wu</name><affiliation wicri:level="1"><nlm:affiliation>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Shaoling" sort="Zhang, Shaoling" uniqKey="Zhang S" first="Shaoling" last="Zhang">Shaoling Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Peng" sort="Wang, Peng" uniqKey="Wang P" first="Peng" last="Wang">Peng Wang</name><affiliation wicri:level="1"><nlm:affiliation>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing, China. wangpeng@njau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Juyou" sort="Wu, Juyou" uniqKey="Wu J" first="Juyou" last="Wu">Juyou Wu</name><affiliation wicri:level="1"><nlm:affiliation>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing, China. juyouwu@njau.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author></analytic><series><title level="j">BMC plant biology</title><idno type="eISSN">1471-2229</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Ammonium Compounds (toxicity)</term><term>Animals (MeSH)</term><term>Arabidopsis (drug effects)</term><term>Arabidopsis (genetics)</term><term>Electrophysiological Phenomena (MeSH)</term><term>Gene Expression Regulation, Plant (drug effects)</term><term>Genes, Plant (MeSH)</term><term>Ion Channels (metabolism)</term><term>Mutation (genetics)</term><term>Nitrates (metabolism)</term><term>Oocytes (drug effects)</term><term>Oocytes (metabolism)</term><term>Phylogeny (MeSH)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plants, Genetically Modified (MeSH)</term><term>Protein Kinases (metabolism)</term><term>Pyrus (drug effects)</term><term>Pyrus (enzymology)</term><term>Pyrus (genetics)</term><term>Xenopus (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Arabidopsis (effets des médicaments et des substances chimiques)</term><term>Arabidopsis (génétique)</term><term>Canaux ioniques (métabolisme)</term><term>Composés d'ammonium (toxicité)</term><term>Gènes de plante (MeSH)</term><term>Mutation (génétique)</term><term>Nitrates (métabolisme)</term><term>Ovocytes (effets des médicaments et des substances chimiques)</term><term>Ovocytes (métabolisme)</term><term>Phylogenèse (MeSH)</term><term>Phénomènes électrophysiologiques (MeSH)</term><term>Protein kinases (métabolisme)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Pyrus (effets des médicaments et des substances chimiques)</term><term>Pyrus (enzymologie)</term><term>Pyrus (génétique)</term><term>Régulation de l'expression des gènes végétaux (effets des médicaments et des substances chimiques)</term><term>Végétaux génétiquement modifiés (MeSH)</term><term>Xenopus (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Ion Channels</term><term>Nitrates</term><term>Plant Proteins</term><term>Protein Kinases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Ammonium Compounds</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Arabidopsis</term><term>Gene Expression Regulation, Plant</term><term>Oocytes</term><term>Pyrus</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Arabidopsis</term><term>Ovocytes</term><term>Pyrus</term><term>Régulation de l'expression des gènes végétaux</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Pyrus</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Pyrus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Arabidopsis</term><term>Mutation</term><term>Pyrus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Arabidopsis</term><term>Mutation</term><term>Protéines végétales</term><term>Pyrus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Oocytes</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Canaux ioniques</term><term>Nitrates</term><term>Ovocytes</term><term>Protein kinases</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="toxicité" xml:lang="fr"><term>Composés d'ammonium</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Electrophysiological Phenomena</term><term>Genes, Plant</term><term>Phylogeny</term><term>Plants, Genetically Modified</term><term>Xenopus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Gènes de plante</term><term>Phylogenèse</term><term>Phénomènes électrophysiologiques</term><term>Végétaux génétiquement modifiés</term><term>Xenopus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>The functional characteristics of SLAC/SLAH family members isolated from Arabidopsis thaliana, poplar, barley and rice have been comprehensively investigated. However, there are no reports regarding SLAC/SLAH family genes from Rosaceae plants.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>In this study, the function of PbrSLAH3, which is predominately expressed in pear (Pyrus bretschneideri) root, was investigated. PbrSLAH3 can rescue the ammonium toxicity phenomenon of slah3 mutant plants under high-ammonium/low-nitrate conditions. In addition, yeast two-hybrid and bimolecular fluorescence complementation assays confirmed that PbrSLAH3 interacts with PbrCPK32. Moreover, when PbrSLAH3 was co-expressed with either the Arabidopsis calcium-dependent protein kinase (CPK) 21 or PbrCPK32 in Xenopus oocytes, yellow fluorescence was emitted from the oocytes and typical anion currents were recorded in the presence of extracellular NO</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSION</b></p><p>We suggest that PbrSLAH3 crossing-talk with PbrCPK32 probably participate in transporting of nitrate nutrition in pear root.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31068146</PMID><DateCompleted><Year>2019</Year><Month>05</Month><Day>17</Day></DateCompleted><DateRevised><Year>2020</Year><Month>02</Month><Day>25</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1471-2229</ISSN><JournalIssue CitedMedium="Internet"><Volume>19</Volume><Issue>1</Issue><PubDate><Year>2019</Year><Month>May</Month><Day>08</Day></PubDate></JournalIssue><Title>BMC plant biology</Title><ISOAbbreviation>BMC Plant Biol</ISOAbbreviation></Journal><ArticleTitle>PbrSLAH3 is a nitrate-selective anion channel which is modulated by calcium-dependent protein kinase 32 in pear.</ArticleTitle><Pagination><MedlinePgn>190</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s12870-019-1813-z</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">The functional characteristics of SLAC/SLAH family members isolated from Arabidopsis thaliana, poplar, barley and rice have been comprehensively investigated. However, there are no reports regarding SLAC/SLAH family genes from Rosaceae plants.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">In this study, the function of PbrSLAH3, which is predominately expressed in pear (Pyrus bretschneideri) root, was investigated. PbrSLAH3 can rescue the ammonium toxicity phenomenon of slah3 mutant plants under high-ammonium/low-nitrate conditions. In addition, yeast two-hybrid and bimolecular fluorescence complementation assays confirmed that PbrSLAH3 interacts with PbrCPK32. Moreover, when PbrSLAH3 was co-expressed with either the Arabidopsis calcium-dependent protein kinase (CPK) 21 or PbrCPK32 in Xenopus oocytes, yellow fluorescence was emitted from the oocytes and typical anion currents were recorded in the presence of extracellular NO<sub>3</sub><sup>-</sup>. However, when PbrSLAH3 alone was injected, no yellow fluorescence or anion currents were recorded, suggesting that anion channel PbrSLAH3 activity was controlled through phosphorylation. Finally, electrophysiological and transgene results showed that PbrSLAH3 was more permeable to NO<sub>3</sub><sup>-</sup> than Cl<sup>-</sup>.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">We suggest that PbrSLAH3 crossing-talk with PbrCPK32 probably participate in transporting of nitrate nutrition in pear root.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Guodong</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Li</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Qian</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Qi</LastName><ForeName>Kaijie</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yin</LastName><ForeName>Hao</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cao</LastName><ForeName>Peng</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tang</LastName><ForeName>Chao</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Xiao</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Shaoling</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Peng</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing, China. wangpeng@njau.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Juyou</ForeName><Initials>J</Initials><Identifier Source="ORCID">http://orcid.org/0000-0001-7479-151X</Identifier><AffiliationInfo><Affiliation>Center of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, No 6. Tongwei Road, Nanjing, China. juyouwu@njau.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>2018YFD0201400</GrantID><Agency>National Key R &D Program of China</Agency><Country></Country></Grant><Grant><GrantID>2014BAD16B03-4</GrantID><Agency>the National Key Technology R & D Program of the Ministry of Science and Technology of China</Agency><Country></Country></Grant><Grant><GrantID>KJQN201926</GrantID><Agency>the Fundamental Research Funds for the Central Universities</Agency><Country></Country></Grant><Grant><GrantID>KYTZ201602</GrantID><Agency>the Fundamental Research Funds for the Central Universities</Agency><Country></Country></Grant><Grant><GrantID>31801842</GrantID><Agency>the National Natural Science Foundation of China</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>05</Month><Day>08</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>BMC Plant Biol</MedlineTA><NlmUniqueID>100967807</NlmUniqueID><ISSNLinking>1471-2229</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D064751">Ammonium Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007473">Ion Channels</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009566">Nitrates</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.-</RegistryNumber><NameOfSubstance UI="D011494">Protein Kinases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.1.-</RegistryNumber><NameOfSubstance UI="C052123">calcium-dependent protein kinase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D064751" MajorTopicYN="N">Ammonium Compounds</DescriptorName><QualifierName UI="Q000633" MajorTopicYN="N">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017360" MajorTopicYN="N">Arabidopsis</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055724" MajorTopicYN="N">Electrophysiological Phenomena</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="N">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007473" MajorTopicYN="N">Ion Channels</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009566" MajorTopicYN="N">Nitrates</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009865" MajorTopicYN="N">Oocytes</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011494" MajorTopicYN="N">Protein Kinases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D031989" MajorTopicYN="N">Pyrus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014981" MajorTopicYN="N">Xenopus</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Anion channel</Keyword><Keyword MajorTopicYN="N">Nitrate</Keyword><Keyword MajorTopicYN="N">Nitrogen</Keyword><Keyword MajorTopicYN="N">PbrSLAH3</Keyword><Keyword MajorTopicYN="N">Phosphorylation</Keyword><Keyword MajorTopicYN="N">Signaling</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>08</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>04</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>5</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>5</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>5</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31068146</ArticleId><ArticleId 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first="Guodong" last="Chen">Guodong Chen</name></noRegion><name sortKey="Cao, Peng" sort="Cao, Peng" uniqKey="Cao P" first="Peng" last="Cao">Peng Cao</name><name sortKey="Chen, Qian" sort="Chen, Qian" uniqKey="Chen Q" first="Qian" last="Chen">Qian Chen</name><name sortKey="Qi, Kaijie" sort="Qi, Kaijie" uniqKey="Qi K" first="Kaijie" last="Qi">Kaijie Qi</name><name sortKey="Tang, Chao" sort="Tang, Chao" uniqKey="Tang C" first="Chao" last="Tang">Chao Tang</name><name sortKey="Wang, Li" sort="Wang, Li" uniqKey="Wang L" first="Li" last="Wang">Li Wang</name><name sortKey="Wang, Peng" sort="Wang, Peng" uniqKey="Wang P" first="Peng" last="Wang">Peng Wang</name><name sortKey="Wu, Juyou" sort="Wu, Juyou" uniqKey="Wu J" first="Juyou" last="Wu">Juyou Wu</name><name sortKey="Wu, Xiao" sort="Wu, Xiao" uniqKey="Wu X" first="Xiao" last="Wu">Xiao Wu</name><name sortKey="Yin, Hao" sort="Yin, Hao" uniqKey="Yin H" first="Hao" last="Yin">Hao Yin</name><name sortKey="Zhang, Shaoling" sort="Zhang, Shaoling" 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